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Julie Legault
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Open Source Microfluidics

These o/s microfluidics devices were made to further explore the lab-on-a-chip idea to culture live organisms on 3d printed parts as part of an MIT course, Open Source Microfluidics for Synthetic Biology.

Traditionally made through stereolithography, microfluidic "lab-on-a-chip" technologies are part of a maturing field of research that involves miniaturizing fluidics systems to mix, move, separate or otherwise process liquids, making it possible to perform bio experiments on devices the size of a stamp, not a room.

As 3d printing these devices is a new area of research, these tests were exploratory and still in progress in the hopes to achieve self-contained microfluidic cell culture systems. Work in this field is explored on a new MIT-designed open-source website, Metafluidics.org,

Research in Collaboration with David Kong, Steven Keating & Jeremy Gam (2014) Matthew Walsh, Daniel S. Kwang Yang Quake & Hsin-ho Huang (2015)

 3D printed Resolution Tests
 MIT logo Reactor  2 input / 1 output mixer   500uL volume in channel
 Manual operation, 2 Channel Mixer
 10 Channel spiral mixer for Golden Gate Reaction   Multiple 2-input spirals for mixing with central chamber for temp. cycling  70ul vol. per spiral / 220ul vol. central chamber (empirical) (474ul vol. spiral / 255ul central theor
 Golden Gate reaction test